Principle of the Assay

p62 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p62 antibody and an p62-HRP conjugate. The assay sample and buffer are incubated together with p62-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p62 concentration since p62 from samples and p62-HRP conjugate compete for the anti-p62 antibody binding site. Since the number of sites is limited, as more sites are occupied by p62 from the sample, fewer sites are left to bind p62-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p62 concentration in each sample is interpolated from this standard curve.

Citations of Mouse Antioncogene P62 Protein ELISA kit

E03A0056 has been referenced in the below publications:

The suppressed autophagy induced by carbon disulfide could be rescued by N-carbamoyl glutamate during the window of embryo implantation in mice.

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