ACES name N-(2-acetamido)-2-aminoethanesulfonic acid. ACES buffer is a zwitterionic (electrically neutral ion) buffer often prepared as a buffer to adjust the pH of a solution in biological research. ACES buffer typically contains sodium hydroxide and sodium chloride. High-purity, high-quality ACES white crystalline powder, the buffer prepared through strict filtration, sterilization and other processes, can be intuitively added to the cell culture medium to adjust the acid-base environment of the medium suitable for cell growth.

ACES Buffer(CAS 7365-82-4) Details

ACES buffer (CAS 7365 82 4) is easily soluble in water, and the water-soluble is colorless and clear. While increasing the clarity of the solution, it can provide a stable acid-base environment for biochemical reactions. ACES buffer and ADA buffer generally belong to the family of acetamido buffers. Nickel, zinc, etc. do not undergo complexation reactions.

When ACES buffer is used to separate proteins by agarose and polyacrylamide gel electrophoresis, it can strictly control the pH range; it can also change the phenotype of α1-antitrypsin during isoelectric focusing of agarose, which is especially suitable for isoelectric protein Bacterial autolysin analysis in focused (IEF) and discontinuous SDS-PAGE systems.

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ACES Buffer(CAS 7365-82-4) Applications

ACES buffer, as an acid-base regulator, is also suitable for the separation, identification and purification of nucleic acids with larger molecular weights in agarose and polyacrylamide gel electrophoresis, using agar or agarose as a support medium;

ACES buffer is widely used in cell research. For example, in preparing buffered charcoal yeast extract agar to isolate bacteria such as Legionella, the effect of adding ACES buffer in air-conditioned cooling water is just suitable for Legionella culture; in washing and heating Lactobacillus plantarum bacterial cells, it is necessary to ACES buffer as a buffer system;

ACES buffer can also be used as a buffer for the analysis of enzymes, such as glucosidase studies, aldehyde dehydrogenase complex X-ray crystallography studies;

ACES buffer is widely used as a separator for protein mechanism resolution.