CAPSO Buffer Cas No.73463-39-5 is an electrically neutral white powdery compound, which is often formulated into CAPSO buffer with sodium hydroxide. Since CAPSO buffer has a pH buffer range of 9.6-11.4, it is more suitable for biochemical and molecular biology research requiring high pH buffers.

CAPS (9.7-11.1) and CABS (10.0-11.4) belong to the cyclohexylamino buffer family.

CAPSO Buffer Details:

CAPSO name 3-(cyclohexylamine)-2-hydroxy-1-propanesulfonic acid. CAPSO buffers have very low metal binding constants and do not form any sizable complexes with metals, making them particularly suitable for studies investigating metal-dependent enzymes. At room temperature of 25℃, the optimum pH value of CAPSO buffer is 10.4. Compared with other propanesulfonic acid buffers, CAPSO buffer has the smallest reaction ability with enzymes and proteins, and the highest solubility in water.

Like CAPS buffer, CAPSO buffer is also an electrically neutral buffer. CAPSO buffers play a key role as buffers in protein studies, such as in studies where specific antibodies are used for gel electrophoresis-treated cell or biological tissue samples to explore the expression of a specific protein in the cell or tissue being analyzed ( Western blotting or immunoblotting experiments), protein sequencing and identification, electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724), CAPS buffer as the pH of the solution required for the study regulator.