Introduction

TMB solutions are chromogenic reagents for peroxidase, used for ELISA techniques. They are composed of 3,3’,5,5’-tetramethylbenzidine (TMB), hydrogene peroxide (H2O2), and proprietary catalyzing and stabilizing agents. It produces a blue product upon interaction with HRP or HRP conjugates without the addition of hydrogen peroxide. The soluble blue product can be quantitated at 650 nm. Sensitivity is greater than classic substrates like OPD and ABTS, with very low background.

Storage

2-8°C avoid light for three years. Do not freeze (Return to room temperature before use).