1. Principle

Ethoxyquin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Ethoxyquin in the fish, shrimp, feed, serum samples.

The coupling antigens are coated on the micro-well stripes. The Ethoxyquin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Ethoxyquin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.

The optical density (OD) value of the sample has a negative correlation with the Ethoxyquin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Ethoxyquin concentration in the cotton sample.

2. Technical specification

Sensitivity: 20ppb Detect time: Approx.45 min

Detect limit:

Feed........................................... 50ppb

Fish,shrimp................................20ppb

Cross-reaction rate:

Ethoxyquin....................................100%

Recovery rate:

Feed.............................................. 85 ± 20%

Fish,shrimp................................. 80 ± 15%