1. Principle

Aflatoxin M1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin M1 in milk, milk powder, cream, cheese, etc.

An antigen with an affinity for Aflatoxin M1 is coated onto microwells. Standard or sample is added to the appropriate well and if Aflatoxin M1 is present it will compete with the coated antigen to bind to Aflatoxin M1 antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The resulting color intensity, has an inverse relationship with the target concentration in the sample. The levels of Aflatoxin M1 in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the Aflatoxin M1 standards provided in the kit.

2. Technical specifications

Sensitivity : 5ng/L (5ppt)

Milk.................................... 5 ppt

Milk powder................... 50 ppt

Fermented milk............. 10 ppt

Recovery rate:

85 ±10%

Cross-reaction rate:

Aflatoxin M1...................100 %

Aflatoxin B1.................. < 1%

AflatoxinG1,G2,M2.....< 0.5%